Galactomannans for Entrapment of Gliomastix murorum Laccase and Their Use in Reactive Blue 2 Decolorization

In the present study, a novel laccase from ascomycete Gliomastix murorum was produced in agro-industrial wastes and entrapped galactomannan beads for Reactive Blue 2 (Rb-2) decolorization. The maximum production agave bagasse-based medium occurred at 72 h (1798.6 UL−1). Entrapped decolorized ˃80% of 0.5 mM Rb-2 without addition redox mediator. Km substrate 1.42 mM, with Vmax 1.19 µmol min−1 laccase. Galactomannan matrices produce stability to acid pH (2–5) temperatures 20–70 °C. Reusability assays showed that could retain efficient decolorization six times. general, used entrapment provides economic advantages large-scale wastewater treatment due its natural origin results.